摘要目的：探讨通过对犬肝组织超声造影定量分析，评价其对肝动脉狭窄诊断的可行性。方法：于犬肝动脉主干使用粗丝线套管钳扎法建立肝动脉狭窄动物模型并经X线血管造影证实。由股静脉弹丸式注入造影剂“全氟显”（0.025～0.03ml/kg）进行肝组织、腹主动脉肝前段及左心室腔超声造影。观察并储存注射造影剂后0～2min的动态图像，利用定量分析QLAB软件进行时间－强度曲线（Time-intensity curve，TIC）分析。分别计算得出肝动脉正常及各级狭窄程度时肝组织、腹主动脉及左心室腔的峰值强度（Peak intensity，PI）、曲线下面积（Area under curve，AUC）、达峰时间（Time to peak，TTP）和曲线下降支斜率（Alpha）。结果：肝动脉狭窄组肝实质增强程度均弱于正常组，肝组织PI值、肝组织PI值与腹主动脉及左心室腔PI比值（PI比值）小于正常组（P＜0.05），且肝组织PI值与肝动脉狭窄程度具有良好的相关性（r=－0.870，P<0.001)。结论：肝组织PI值与PI比值是判断肝动脉血流异常的最敏感指标，能够评价肝动脉狭窄程度。
Abstract：Objective: To investigate the feasibility of hepatic artery stenosis with quantitative analyzing of contrast enhanced ultrasound in perfusion of canine liver tissue. Methods: Canine hepatic artery stenosis models were established successfully by thick thread pipe forceps ligature and be validated using X-ray angiography. Sonographic images of liver tissue, abdominal aorta(AO) in front of liver and left ventricle(LV) were captured continuously for a total of 2 minutes after administration of a bolus injection of ultrasound contrast agent(Perfluoropropare-albumin microsphere, 0.025～0.03ml/kg) by femoral vein. Sonographic liver tissue perfusion images were converted into a liver perfusion time-intensity curve(TIC) by the quantitative analyzing software. Furthermore, the respectively calculated parameters such as peak intensity(PI), area under curve(AUC), time to peak(TTP) and Alpha were compared with normal hepatic artery and different stenotic level of hepatic artery, liver tissue, AO and LV. Results: In the hepatic artery stenosis group, they exhibited liver parenchyma intensive level were lower than control group. The PI of liver tissue and the PI ratio of liver tissue/AO or LV were lower than control group（P＜0.05）. In addition, the PI of liver tissue was negatively correlated with hepatic artery stenotic level(r=－0.870, P＜0.001). Conclusions: The PI and PI ratio are most sensitive indices of detect hepatic arterial hemodynamic variation and it can be used to evaluate the stenotic degree of hepatic artery.
2008, Vol. 19 
