Abstract: Objective: To explore the value of contrast-enhanced ultrasonography(CEUS) in the diagnosis of retroperitoneal arterial hemorrhage. Methods: Five active bleeding sites were created in the external iliac artery of five rabbits. CEUS was used to observe the feature of active bleeding 2 min, 10 min and 40 min after the arterial injury. DSA was also used at 20 min and 30 min after the arterial injury, as a gold standard. Results: 2 min after the arterial injury, CEUS showed active bleeding sites with the feature of jet-like and high flow at the bleeding point. At 10 min and 40 min after the arterial injury, CEUS showed the feature of seeping-out and lower speed flow than before. The results were consistent with DSA. Conclusion: CEUS can display active bleeding of retroperitoneal arterial injury, and it will have good prospects for clinical application.